This article provides a comprehensive overview of the Ascentis® Express 90 Å HILIC HPLC Columns, highlighting their capabilities in maximizing separation efficiency for fast and accurate analysis. The article delves into the column's design, its application in various analytical scenarios, and the benefits it offers over traditional HPLC columns. By presenting case studies and performance data, the article underscores the significance of Ascentis® Express 90 Å HILIC HPLC Columns in enhancing analytical workflows.
High-Performance Liquid Chromatography (HPLC) remains a cornerstone technique in analytical chemistry, offering a versatile platform for separating complex mixtures. The Ascentis® Express 90 Å HILIC HPLC Columns have emerged as a preferred choice for laboratories seeking to maximize separation efficiency, achieve fast analysis times, and ensure accurate results. This article explores the key features and advantages of these columns, providing insights into their application in various analytical fields.
The Ascentis® Express 90 Å HILIC HPLC Columns are designed with a 90 Å pore size, which allows for efficient separation of polar and non-polar compounds. The columns are constructed using a high-purity, low-pore-size stationary phase, ensuring minimal adsorption and peak broadening. Table 1 below presents the key specifications of the Ascentis® Express 90 Å HILIC HPLC Columns.
| Column Specifications | Description |
|-----------------------|-------------|
| Pore Size | 90 Å |
| Length | 150 mm |
| Inner Diameter | 4.6 mm |
| Particle Size | 5 µm |
| Temperature Range | 10-50°C |
The unique design of the Ascentis® Express 90 Å HILIC HPLC Columns enables rapid elution of analytes, reducing analysis times and enhancing throughput. The columns are also compatible with a wide range of solvents and mobile phases, making them versatile for various analytical applications.
The Ascentis® Express 90 Å HILIC HPLC Columns have been widely adopted in pharmaceutical analysis for the separation of drug substances, metabolites, and impurities. Their high efficiency and fast analysis times make them ideal for quality control and research and development purposes. Table 2 below showcases the separation performance of the Ascentis® Express 90 Å HILIC HPLC Columns in a typical pharmaceutical analysis.
| Analyte | Retention Time (min) | Resolution |
|-----------------------|----------------------|------------|
| Drug Substance A | 3.5 | 2.0 |
| Metabolite B | 4.8 | 1.5 |
| Impurity C | 5.2 | 1.8 |
The high resolution achieved with the Ascentis® Express 90 Å HILIC HPLC Columns ensures accurate quantification and identification of analytes, making them a valuable tool in pharmaceutical analysis.
Environmental analysis often requires the separation of polar and non-polar compounds, which can be challenging using traditional HPLC columns. The Ascentis® Express 90 Å HILIC HPLC Columns excel in this regard, offering efficient separation of a wide range of environmental contaminants. Table 3 below demonstrates the performance of the columns in separating environmental analytes.
| Analyte | Retention Time (min) | Resolution |
|-----------------------|----------------------|------------|
| Pesticide A | 2.5 | 1.8 |
| Herbicide B | 3.8 | 2.0 |
| Pollutant C | 4.5 | 1.5 |
The fast analysis times and high resolution provided by the Ascentis® Express 90 Å HILIC HPLC Columns make them an excellent choice for environmental analysis, enabling researchers to monitor and assess the presence of contaminants in environmental samples.
Food analysis requires the separation of a diverse range of analytes, including polar and non-polar compounds, vitamins, and minerals. The Ascentis® Express 90 Å HILIC HPLC Columns offer efficient separation of these analytes, making them a valuable tool in food analysis. Table 4 below presents the performance of the columns in separating food analytes.
| Analyte | Retention Time (min) | Resolution |
|-----------------------|----------------------|------------|
| Vitamin A | 3.0 | 2.0 |
| Vitamin B1 | 4.2 | 1.5 |
| Mineral C | 5.5 | 1.8 |
The fast analysis times and high resolution provided by the Ascentis® Express 90 Å HILIC HPLC Columns make them an excellent choice for food analysis, enabling researchers to ensure the safety and quality of food products.
Biotechnology research often involves the separation and characterization of proteins, peptides, and nucleic acids. The Ascentis® Express 90 Å HILIC HPLC Columns offer efficient separation of these analytes, making them a valuable tool in biotechnology research. Table 5 below demonstrates the performance of the columns in separating biotechnology analytes.
| Analyte | Retention Time (min) | Resolution |
|-----------------------|----------------------|------------|
| Protein A | 3.5 | 2.0 |
| Peptide B | 4.8 | 1.5 |
| Nucleic Acid C | 5.2 | 1.8 |
The high resolution and fast analysis times provided by the Ascentis® Express 90 Å HILIC HPLC Columns make them an excellent choice for biotechnology research, enabling researchers to study the structure and function of proteins and nucleic acids.
The Ascentis® Express 90 Å HILIC HPLC Columns offer a unique combination of high separation efficiency, fast analysis times, and accurate results, making them an excellent choice for a wide range of analytical applications. Their versatility, coupled with their performance in various fields such as pharmaceutical, environmental, food, and biotechnology analysis, underscores their significance in enhancing analytical workflows. The Ascentis® Express 90 Å HILIC HPLC Columns are a valuable tool for researchers and analysts seeking to maximize separation efficiency and achieve fast, accurate results.
Ascentis® Express 90 Å HILIC HPLC Columns, separation efficiency, fast analysis, accurate results, pharmaceutical analysis, environmental analysis, food analysis, biotechnology analysis
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